Journal: Cell Proliferation

Article Title: Do mesenchymal stem cells play a role in vocal fold fat graft survival?

doi: 10.1111/j.1365-2184.2008.00533.x

Figure Lengend Snippet: Primer sequences used for RT‐PCR. The primers were constructed on the basis of published human sequences, and selected using version 1.5 of Primer Express software available from Applied Biosystems

Article Snippet: The same population was also stained with conjugated mouse–antihuman antibodies VE‐cadherin FITC (Chemicon; 1 : 25) and CD146 FITC (Chemicon; 1 : 100).

Techniques: Construct, Software, Sequencing

Journal: Cell Proliferation

Article Title: Do mesenchymal stem cells play a role in vocal fold fat graft survival?

doi: 10.1111/j.1365-2184.2008.00533.x

Figure Lengend Snippet: (a) Morphology of ADMSCs after exposure to endothelial conditioning medium (×20 magnification). Fluorescence image of ADMSCs after immunostaining with mouse–antihuman monoclonal antibodies for von Willebrand factor (b) and VE‐cadherin (c). (d) and (e) are the negative controls. Nuclei counterstained with DAPI (blue) (×60 magnification).

Article Snippet: The same population was also stained with conjugated mouse–antihuman antibodies VE‐cadherin FITC (Chemicon; 1 : 25) and CD146 FITC (Chemicon; 1 : 100).

Techniques: Fluorescence, Immunostaining

Journal: Nature Materials

Article Title: Parenchymal and stromal tissue regeneration of tooth organ by pivotal signals reinstated in decellularized matrix

doi: 10.1038/s41563-019-0368-6

Figure Lengend Snippet: Fig. 4 | Alx3-restored adult MSC-induced angiogenesis and VEGF signalling. a,d, Angiogenesis in regenerated tissues with magnifications. d, native dentin. b,e, Human von Willebrand factor immunohistochemistry with magnifications. Green arrowheads, endothelial cells of human origin (e); black arrowheads, endothelial cells of host (mouse) origin (e); red arrowheads chimeric blood vessels (e). c,f, Human mitochondria staining; red arrowheads, human cells; black arrowheads, host (mouse) cells. a–f, n = 5 independent biological samples. g, Blood vessel (bv) quantification. n = 5 independent biological samples, presented as the mean ± s.d. P values calculated by one-way ANOVA with Bonferroni: **P < 0.01, ***P < 0.001. h, CCK8 of HUVECs treated with conditioned medium (CM) by vector control or Alx3-restored MSCs. n = 4 independent biological samples, presented as the mean ± s.d. P values calculated by one-way ANOVA with Bonferroni: *P < 0.05). i–o, Transwell migration assay by Alx3 conditioned medium or with neutralizing antibody or VEGFR2 inhibitor (i–n) and quantification (o). n = 4 independent biological samples, presented as mean ± s.d. P values calculated by one-way ANOVA with Bonferroni: *P < 0.05, **P < 0.01, ***P < 0.001). p, Mouse VEGF A promoter luciferase reporter with two Alx3-binding elements in the dashed red boxes (left) and VEGF luciferase assay on Alx3 transfection (right). n = 3 independent biological samples, presented as median with range. P values calculated by Kruskal–Wallis tests: *P < 0.05). BE, binding element. q,r, VEGF production and p-VEGFR2 activation by western blot (q) and quantification (r). n = 4 independent biological samples, presented as the mean ± s.d. P values calculated by one-way ANOVA with Bonferroni: *P < 0.05, **P < 0.01, ***P < 0.001. s–x: The tube formation of HUVECs treated by Alx3 conditioned medium with or without NAb or VEGFR2 inhibitor. n = 3 independent biological samples. y, Tube length quantification. n = 3 independent biological samples, presented as the median with range. P values calculated by Kruskal–Wallis tests: *P < 0.05, **P < 0.01. z, rtPCR of VE-cadherin, PECAM1, VEGF and Flk1. n = 4 independent biological samples, presented as the mean ± s.d. P values calculated by one-way ANOVA with Bonferroni: *P < 0.05, **P < 0.01, ***P < 0.001).

Article Snippet: The 1.6 kB VEGF luc plasmid was a gift from P. D’Amore (Addgene plasmid no. 29667).

Techniques: Immunohistochemistry, Staining, Plasmid Preparation, Control, Transwell Migration Assay, Luciferase, Binding Assay, Transfection, Activation Assay, Western Blot, Reverse Transcription Polymerase Chain Reaction

Journal: Developmental cell

Article Title: Hematopoietic Reprogramming in vitro Informs in vivo Identification of Hemogenic Precursors to Definitive Hematopoietic Stem Cells

doi: 10.1016/j.devcel.2016.02.011

Figure Lengend Snippet: E10.5 (left panels) and E12.5 (right panels) placentas were dissociated to a single cell suspension and analyzed. Prom1+Sca1+CD34+ cells are displayed for (A) FCS/SSC, CD49f and (B) CD31, CD105, VE-Cadherin, Tie2, CD41 and cKit expression. Red boxes highlight the phenotype Prom1+Sca1+CD45− cells.

Article Snippet: Ao, Aorta. ( D ) Fluidigm qRT-PCR analysis of Prom1 , Ly6a and CD45 in E10.5 AGM+VU endothelial (EC: CD144+(VE-Cadherin)Ter119−CD45−CD41−23GFP−), hemogenic endothelium (HE: CD144+Ter119−CD45−CD41−23GFP+) and HSPCs (CD144+Ter119−CD45−CD41+23GFP+) (mean ± SD, n=3).

Techniques: Expressing

Journal: Developmental cell

Article Title: Hematopoietic Reprogramming in vitro Informs in vivo Identification of Hemogenic Precursors to Definitive Hematopoietic Stem Cells

doi: 10.1016/j.devcel.2016.02.011

Figure Lengend Snippet: Placentas from (A) E10.5, (B) E11.5 and (C) E12.5 were stained with antibodies against CD133 (Prom1, green). The left panels show composite low magnification pictures of transversal sections of placentas. The region of the labyrinth that contains Prom1+ cells is highlighted (white circles).

Article Snippet: Ao, Aorta. ( D ) Fluidigm qRT-PCR analysis of Prom1 , Ly6a and CD45 in E10.5 AGM+VU endothelial (EC: CD144+(VE-Cadherin)Ter119−CD45−CD41−23GFP−), hemogenic endothelium (HE: CD144+Ter119−CD45−CD41−23GFP+) and HSPCs (CD144+Ter119−CD45−CD41+23GFP+) (mean ± SD, n=3).

Techniques: Staining

Journal: Developmental cell

Article Title: Hematopoietic Reprogramming in vitro Informs in vivo Identification of Hemogenic Precursors to Definitive Hematopoietic Stem Cells

doi: 10.1016/j.devcel.2016.02.011

Figure Lengend Snippet: (A) Hemogenic PS34 cells from E10.5 and E12.5 placentas were fractioned into CD45−cKit−, CD45−cKit+ and CD45+cKit+ populations. HSPCs and mature blood cells (MBC) were also sorted for comparison.

Article Snippet: Ao, Aorta. ( D ) Fluidigm qRT-PCR analysis of Prom1 , Ly6a and CD45 in E10.5 AGM+VU endothelial (EC: CD144+(VE-Cadherin)Ter119−CD45−CD41−23GFP−), hemogenic endothelium (HE: CD144+Ter119−CD45−CD41−23GFP+) and HSPCs (CD144+Ter119−CD45−CD41+23GFP+) (mean ± SD, n=3).

Techniques:

Journal: Developmental cell

Article Title: Hematopoietic Reprogramming in vitro Informs in vivo Identification of Hemogenic Precursors to Definitive Hematopoietic Stem Cells

doi: 10.1016/j.devcel.2016.02.011

Figure Lengend Snippet: (A) E10.5 AGM+VU regions were isolated, dissociated to a single cell suspension and analyzed by flow cytometry. PS34CD45− and PS34CD45+ cells are gated and displayed for the expression of the 23GFP Runx1 reporter. Percentages are shown as mean ± SD, n=3.

Article Snippet: Ao, Aorta. ( D ) Fluidigm qRT-PCR analysis of Prom1 , Ly6a and CD45 in E10.5 AGM+VU endothelial (EC: CD144+(VE-Cadherin)Ter119−CD45−CD41−23GFP−), hemogenic endothelium (HE: CD144+Ter119−CD45−CD41−23GFP+) and HSPCs (CD144+Ter119−CD45−CD41+23GFP+) (mean ± SD, n=3).

Techniques: Isolation, Flow Cytometry, Expressing

Journal: Developmental cell

Article Title: Hematopoietic Reprogramming in vitro Informs in vivo Identification of Hemogenic Precursors to Definitive Hematopoietic Stem Cells

doi: 10.1016/j.devcel.2016.02.011

Figure Lengend Snippet: (A) E12.5 PS34CD45− cells were co-cultured on OP-9 in the presence of cytokines (SCF, Fltl3L, IL-3, IL-6 and TPO) for 4 days. FACS analysis shows the generation of CD45+CD34+cKit+ cells that were tested for clonogenic activity.

Article Snippet: Ao, Aorta. ( D ) Fluidigm qRT-PCR analysis of Prom1 , Ly6a and CD45 in E10.5 AGM+VU endothelial (EC: CD144+(VE-Cadherin)Ter119−CD45−CD41−23GFP−), hemogenic endothelium (HE: CD144+Ter119−CD45−CD41−23GFP+) and HSPCs (CD144+Ter119−CD45−CD41+23GFP+) (mean ± SD, n=3).

Techniques: Cell Culture, Activity Assay

Journal: International Journal of Molecular Sciences

Article Title: Endoglin Promotes Myofibroblast Differentiation and Extracellular Matrix Production in Diabetic Nephropathy

doi: 10.3390/ijms21207713

Figure Lengend Snippet: Endoglin is co-localized with the myofibroblast marker α-SMA. Kidney biopsy samples from non-diabetic controls ( A , E , I , M ) and patients with DN ( B – D , F – H , J – L , N – P ) were co-immunostained for endoglin (red) and the endothelial cell marker CD31 ( A – D ), the macrophage marker CD68 ( E – H ), the fibroblast marker vimentin ( I – L ), or the myofibroblast marker α-SMA ( M – P ). Note the co-localization of endoglin and the endothelial marker CD31 in blood vessels, the co-localization of endoglin and the fibroblast marker vimentin, as well as the co-localization of endoglin and the myofibroblast marker α-SMA, particularly in the patients with DN ( H ). The scale bars represent 50 µm.

Article Snippet: The following primary antibodies were used: Goat anti-human endoglin (1:800, R&D systems), mouse anti-human CD31 (IgG1, clone JC70A, 1:200; Dako, Glostrup, Denmark) to stain endothelial cells, mouse anti-human CD68 (IgG1, clone KP1, 1:2000; Dako) to stain macrophages, mouse anti-human vimentin (IgG2a, clone Vim 3B4, 1:750; Dako) to stain fibroblasts, and mouse anti-human α-SMA (IgG2a, clone 1A4, 1:400; Dako) to stain myofibroblasts.

Techniques: Marker