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Image Search Results
Journal: International journal of oncology
Article Title: Efficacy of schedule-dependent metronomic S-1 chemotherapy in human oral squamous cell carcinoma cells.
doi: 10.3892/ijo.2013.1950
Figure Lengend Snippet: Figure 3. Expression of (A) EGR-1, (B) TSP-1 and (C) VEGF protein in HSC2 tumors. Tumors were either untreated (control) or treated with different treatment regimens of S-1 (6.9 mg/kg). The expression of EGR-1, TSP-1 and VEGF was detected with immunohistochemical staining. Dark brown staining on the nuclei is positive (original magnification, x200).
Article Snippet: These sections were fixed and then processed for immunostaining using the antiEGR-1 mouse monoclonal antibody (Santa Cruz Biotechnology Inc., Santa Cruz, CA, USA), anti-TSP-1 mouse monoclonal antibody (Santa Cruz),
Techniques: Expressing, Control, Immunohistochemical staining, Staining
Journal: International journal of oncology
Article Title: Efficacy of schedule-dependent metronomic S-1 chemotherapy in human oral squamous cell carcinoma cells.
doi: 10.3892/ijo.2013.1950
Figure Lengend Snippet: Figure 7. Anti-tumor effects of metronomic 5-FU in vitro. (A) Growth inhibitory effect of 0.5 µg/ml 5-FU on HSC2 cells. HSC2 cells (5x103 cells per well) were seeded on 96-well plates and exposed to 5-FU according to the schedule shown in Fig. 6. At the end of each treatment regimen, cells were counted using a microplate reader. Each data point represents the mean of three independent determinations. *p<0.05 when compared with that of control by one-way ANOVA. (B) Expression of EGR-1, TSP-1, VEGF and CD44 protein in HSC2 cell lysates. HSC2 cells were treated according to the schedule shown in Fig. 6, and the whole cell lysates were obtained. Equal amount of cellular protein (50 µg) was subjected to SDS-PAGE, followed by western blot analysis for EGR-1, TSP-1, VEGF and CD44. α-tubulin was used as a control for equal protein load.
Article Snippet: These sections were fixed and then processed for immunostaining using the antiEGR-1 mouse monoclonal antibody (Santa Cruz Biotechnology Inc., Santa Cruz, CA, USA), anti-TSP-1 mouse monoclonal antibody (Santa Cruz),
Techniques: In Vitro, Control, Expressing, SDS Page, Western Blot